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The reduction of tropinone is mediated by NADPH-dependent reductase enzymes, which have been characterized in several plant species.[146] These plant species all comprise two types of the reductase enzymes, tropinone reductase I and tropinone reductase II.

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TRI generates tropine and TRII provides pseudotropine. As a consequence of differing kinetic and pH/activity traits of your enzymes and from the 25-fold bigger action of TRI over TRII, virtually all the tropinone reduction is from TRI to form tropine.[147]

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